PCR Modifications

• Type of PCR Modification wherein more than one primer pair is added to PCR that are primed simultaneously.

  Multiplex PCR
• Advantage of multiplex PCR?

  Many in one go
• Disadvantage of multiplex PCR?

  Inefficient and with cross-reactivity
• Type of PCR modification that uses two pairs of primers that are used to amplify a single target in separate PCR programs.

  Nested PCR
• Advantage of nested PCR?

  Increased sensitivity
• Disadvantage of nested PCR?

  Time consuming
• Types of PCR modifications?

  Multiplex PCR

  Nested PCR

  Reverse Transcriptase PCR (RT-PCR)

  Real-Time PCR (rt-PCR)
• Type of PCR modification wherein the starting material is RNA and converted to DNA by_____. It is used in gene expression studies.

  Reverse Transcriptase PCR
• It is a type of PCR modification that detects how much of the target sequence is present usually by fluorescence. It is useful in detecting gene copy numbers, viral load, tumor load, and effects of treatment.

  Real-time PCR
• It is a type of assay that replaced Ethidium Bromide(EtBr). It is highly specific and robust fluorescence. It is less toxic than EtBr, and cheaper than TaqMan?

  SYBR Green 1 Assay
• It is a type of assay wherein fluorescent occurs once the dye is separated from the quencher. The probe makes it more specific than SYBR Green, and it produces higher quality results.

  TaqMan Assay