Steps for Sputum Collection
2. Ask client to do DBE and CE, then cough up 1 to 2 tbsp, or 15-30 mL of sputum
3. Wear gloves to avoid direct contact with the sputum. Wear a mask capable of filtering droplet nuclei
4. Ask the client to expectorate (spit out) the sputum into the specimen container. Make sure the sputum does not contact the outside of the container. If the outside of the container does become contaminated, wash it with a disinfectant
5. Offer mouthwash or mouthcare after sputum collection
6. Label and transport specimen to the laboratory. Bacterial cultures should be started immediately before any contaminating organisms can grow, multiply, and produce false results