The process by which a drug reversibly leaves the bloodstream and enters the interstitium (extracellular fluid) and the tissues
Drug distribution
It is a Passive process in which the driving force is the concentration gradient between the involved compartments i.e. Blood and extravascular tissue
It occurs by diffusion of free drug until equilibrium is attained
Phases of drug distribution
1. Initial distribution phase
2. Redistribution
Initial distribution phase
Mainly determined by cardiac output and regional blood flow
Drugs are initially distributed to tissues with the highest blood flow (heart, brain, lungs, kidneys, and liver)
Enables a rapid onset of action of the drugs affecting these organs
In hypovolemic states
The effect of the drug increases so the dose must be reduced
Redistribution
Highly lipid soluble drugs get initially distributed to organs with high blood flow i.e. brain, heart, kidney, etc.
Later, less vascular but more bulky tissues (skeletal muscles, skin, adipose tissues) take up the drug which leads to fall in the plasma concentrations and the drug is withdrawn from the highly perfused sites
If the site of action of the drug was in one of the highly perfused organs, redistribution results in termination of drug action
Greater the lipid solubility of the drug, the faster is its redistribution
The variation in blood flow between the two phases
Partly explains the short duration of hypnosis produced by an IV bolus of Propofol
High blood flow combined with the high lipophilicity nature of propofol
Leads to rapid distribution in the CNS and produces anaesthesia
A subsequest slower distribution to the skeletal muscles and adipose tissues
Lowers the plasma concentration, causing the drug to diffuse out of the CNS, down the concetration gradient, and consciousness is regained
Oral diazepam exerts a short hypnotic action lasting 6-8 hours
Due to redistribution despite their elimination t½ of > 30 hr
When the same drug is given repeatedly or continuously over long periods
The low perfusion-high capacity sites get progressively filled up and the drug becomes longer acting
Patterns of drug distribution
Non-uniform distribution
Plasma
Throughout body water
Sequestered in specific body parts/tissues
Non-uniform distribution
Most common pattern, largely determined by ability of the drug to cross membranes and their lipid/water solubility
Plasma
Drugs remain within the vascular system as they have large size or are highly bound to plasma protein, hence cannot cross capillary wall easily
Throughout body water
Low molecular weight water soluble compounds like ethanol and some sulphonamide become uniformly distributed throughout the body water
Sequestered in specific body parts/tissues
Concentrated specifically in one or more tissues that may or may not be the site of action (few drugs)
Sequestered in specific body parts/tissues
Iodine is concentrated in the thyroid gland
Chloroquine may be present in the liver at a concentration 1000x more than that in plasma
Tetracycline is almost irreversibly bound to bone and developing teeth
Factors affecting drug distribution
Tissue permeability to drugs
Physicochemical properties of the drug
Physiological barriers
Physiological barriers
Simple Capillary Endothelial Barrier
Simple Cell Membrane Barrier
Blood-Brain Barrier
Blood-CSF Barrier
Blood-Placental Barrier
Blood-Testis Barrier
The membrane of capillaries that supply blood to most tissues is not a perfect barrier because all drugs, whether ionized or not ionized, with a molecular weight <600 Daltons, diffuse through the capillary endothelium
Drugs that are bound to blood componentns are restricted because they have a large molecular size of complex
Blood-Brain Barrier
Made up of a tight junction of capillary cells unlike the capillaries of other organs making it difficult for drugs to pass through
Blood-CSF Barrier
Similar permeability to the BBB- highly lipid soluble drugs can easily cross this barrier, however, moderately soluble and ionized drugs may permeate slowly
The brain is consequently inaccessible to many drugs whose lipid solubility is insufficient to allow penetration of the blood–brain barrier
Inflammation can disrupt the integrity of the blood–brain barrier, allowing normally impermeant substances to enter the brain; consequently, penicillin can be given intravenously (rather than intrathecally) to treat bacterial meningitis (which is accompanied by intense inflammation)
Blood-Placental Barrier
It is the barrier between the maternal and fetal blood vessels, separated by fetal trophoblast basement membrane and endothelium
Highly lipid soluble drugs and those with molecular weight <1000 Daltons, cross the barrier by simple diffusion
P Glycoprotiens form a functional barrier between maternal and fetal blood circulation in the placenta thus protecting the fetus from exposure to the xenobiotics during pregnancy
Drugs safe in pregnancy
Water soluble
Large size
Highly protein bound
Drugs safe in pregnancy
Propylthiouracil (PTU) is chosen over methimazole due to its highly protein bound feature
Phenobarbitone is considered safe compared to phenytoin, carbamazepine and valproic acid due to its high protein bound feature
Blood-Testis Barrier
Located at the Sertoli-Sertoli junction, which is a tight junction between the Sertoli cells and acts as a barrier which restricts the passage of drugs to the spermatocytes and spermatids
Extravascular/Tissue protein binding
60% of the drug is binded by the plasma protein
40% can pass the cell membrane and bind directly to the organs
Plasma protein binding
Forms a reservoir of drug but only the free(unbound) drug is available to the tissue to exert the therapeutic effect
Plasma albumin is a major drug binding protein and may act as a reservoir
Alpha Glycoprotien, Lipoprotien, Globulin are other plasma proteins that bind drugs
One drug can bind to more than one site of albumin
More than one drug can bind to the same site
Warfarin and Sulfonylurea
Both these drugs bind to plasma albumin and each of these drugs has been noted to be more than 99% bound
They compete for binding sites on plasma albumin
Since only an unbound drug is active, any decrease in binding would result into an increased pharmacologic effect
Miscellaneous factors affecting drug distribution
Volume of distribution
The theoretical volume of fluid into which the total drug administered would have to be diluted to produce the concentration in plasma at a steady state
Provides a reference for the plasma concentration expected for a given dose but provides little information about the specific pattern of distribution
Each drug is uniquely distributed in the body
Volume of distribution
If 1000 mg of a drug is given and the subsequent plasma concentration is 10 mg/L, that 1000 mg seems to be distributed in 100 L (dose/volume = concentration; 1000 mg/x L = 10 mg/L; therefore, x= 1000 mg/10 mg/L = 100 L)
Typical liquid volumes in average 70kg man
Total water 60% (50-80%) 42L
Intracellular volume 40% 28L
Extracellular volume 20% 14L
Plasma volume 4% 3L
Blood volume 8% 5.5L
For a drug that is highly tissue-bound
Very little drug remains in the circulation; thus, plasma concentration is low and volume of distribution is high