Sediment Stains

• Sediment stains increases the overall what of sediment elements?

  VISIBILITY
• What is the most commonly used sediment stain?

  Sternheimer-Malbin Stain
• Sternheimer-malbin stain delineates the structure and contrasting colors of what 2 organelles?

  nucleus and cytoplasm
• Sternheimer-Malbin Stain stains what types of urinary sediments?

  WBCs, epithelial cells, and CASTS
• What are the 2 components of Sternheimer-malbin stain?

  Crystal violet and safranin O
• What are the 2 brands of Sternheimer-malbin stain?

  KOVA and Sedi Stain
• What percentage of Toluidine blue is used?

  0.5%
• Toluidine blue enhances the detail of what?

  nucleus
• Toluidine blue differentiates what 2 urinary sediments?

  WBCs and RTE cells
• What sediment stain is used to lyse RBCs and enhance WBCs nuclei?

  2% acetic acid
• 2% acetic acid distinguishes RBCs from what?

  WBCs, yeast, oil droplets, and crystals
• What is the most common constituent that causes interference for RBC?

  Crystals
• What are the 2 lipid stains?

  Oil red O and Sudan III
• Lipid stains stain TRIGLYCERIDES and neutral fats what color?

  Orange-red
• SUDAN III do not stain what?

  Cholesterol
• What stain identifies free fat droplets and lipid-containing cells and casts?

  Oil Red O and Sudan III
• What stain differentiates gram-positive and gram-negative (nitrate-converting) bacteria?

  Gram Stain
• What stain identifies BACTERIAL CASTS?

  Gram stain
• What color is gram-positive bacteria?

  Blue
• What color is gram-negative bacteria?

  red
• What stains identifies urinary EOSINOPHILS?

  Hansel stain and Wright's stain
• Methylene blue and eosin Y stains eosinophilic granules.
• What stain stains structures containing IRON?

  Prussian blue
• Prussian blue stain identifies YELLOW-BROWN GRANULES of HEMOSIDERIN in CELLS and CASTS
• Hemoglobin turns into what?

  Hemosiderin