The production of pre-mRNA from a DNA strand formed by complementary base pairing
What is translation?
Using the mRNA to produce a polypeptide using amino acids, at the ribosome
What is splicing?
The removal of introns from pre-mRNA
How does transcription work?
The enzyme DNA helicase unzips tye DNA along the required gene (breaking the H bonds)
RNA bases join to the exposed DNA bases on the template strand according to complementary base pairing. (In mRNA T is replaced with U). This is catalysed by the enzyme RNA polymerase
mRNA leaves the nucleus via. a nuclear pore and attaches to a ribosome in the cytoplasm
How does Translation work?
The ribosome moves along the mRNA strand reading the code 3 bases at a time. 3 bases = 1 codon
tRNA molecules contain an anticodon (set of 3 bases) at one end and an amino acid specific to anticodon at the other end. tRNA anticodons bind with the complementary mRNA codon
This brings the amino acids together in the correct order to produce the primary structure of the protein. Peptide bonds then form bewgeen the amino acids by condensation and the tRNA molecules are releases
