By 2 billion years ago, O2 levels began to build up in the atmosphere. The presence of O2 had a profound impact on life on Earth - it is toxic to organisms that don't have protective mechanisms, many died as O2 levels built up, it creates an oxidizing atmosphere which can destroy precious reduced organic molecules, some anaerobic organisms survive only in environments with little to no oxygen, some evolved adaptations to the presence of oxygen, and some organisms developed means to use O2 in respiration to extract more energy from foods (aerobic respiration).