Kineticenergy of molecules increases so they move around rapidly and collide more often. At some point the enzyme is denatured, they lose the shape of their activesite and cannot bind to their substrate
How does pH affect enzyme action?
Deviation from optimum pH breaks hydrogen bonds which alters the shape of the substrate. It also may cause the bonds in the tertiary structure to break
How does enzyme concentration affect enzyme action?
As enzyme concentration increases so does the rate of reaction
How does substrate concentration affect enzyme action?
As substrate concentration increases, so will rate of reaction. It will keep increasing until there are no more active sites available
