Microscopes

• Magnification is how many times larger an image is
• Resolution is the minimum distant between 2 objects in which they can still be reviewed seperately
• Optical Microscopes use light
• Optical Microscopes have limited resolution of 0.2um, meaning only eukaryotic cells and large organelles are viewed
• Optical Microscopes have a maximum magnification of x1500
• Electron Microscopes use electron beams
• Electron Microscopes have a maximum resolution of 0.00002um / 0.2nm, and can observe small organelles
• Electron Microscopes have a maximum magnification of x1500000
• Transmission EMs are denser, darker images
• Transmission EMs have high resolution and can view internal structures
• Transmission EMs cant be used on living specimen, and sample has to be thin enough to allow electrons to pass through
• Scanning EMs create 3D imaging on 3D specimen
• Scanning EMs can view external structural views and have low resolution
• Eye Piece Graticules measure the size of objects, but must be calibrated each time
• Steps of Drawing Cells

  • Title
  • Magnification Scale
  • Single Lines
  • No Shading
  • Line Labels that connect Directly