Monoclonal Antibodies

• Direct Monoclonal Therapy treat cancer by binding to complementary site, preventing chemicals binding causing uncontrollable division
• Indirect Monoclonal Therapy treat cancer with drugs attached, killing the cell - known as 'bullet drugs'
• ELISA Test:

  • 1st: mobile antibody complementary to antigen with a colour dye
  • 2nd: antibody is complementary to the antigen and is immobilised
  • 3rd: antibody is complementary to 1st antibody and is immobilised

  
• ELISA Test Alternative:

  • Test Sample added to beaker
  • Wash to remove any unbound sample
  • Add antibody complementary to bound antigen
  • Wash to remove any unbound antibody
  • Add second antibody complementary to the first antibody, holding an enzyme
  • Substrate for the enzyme is added, turning from colourless to coloured
  • Intensity of colour indicates quantity present