culturing microorganisms

• give ways of growing bacteria

  nutrient broth and agar gel plate
• describe growth conditions of bacteria

  sufficient nutrient supply and suitable temperature
• describe use of uncontaminated cultures of microorganisms

  investigate action of antibiotics and disinfectants
• give examples of aseptic techniques

  sterilisation, taped lid, upside down storage and incubation at 25°C
• describe sterilisation

  sterilise petri dish and culture medium before use and inoculating loop by passing through flame to kill unwanted microorganisms
• describe taped lid

  prevent microorganisms entering from surroundings
• describe upside down storage

  prevent condensation drops falling on surface
• describe incubation at 25°C

  prevent growth of harmful microorganisms