bio 101 m6 pt 2

• S-phase - where dna synthesis occurs
• Initiation - Recognition of position/s on the DNA molecule where replication will begin
• Elongation - Synthesis of the complementary daughter strand at the replication fork
• Termination - Completion of the process
• Prokaryotes/viruses - single origin (oriC)
• Eukaryotes - multiple origin
• OriC made up of 245 bp
• OriC made up of 2 parts: 13-nuc repeat motif (3x) and 9-nuc repeat motif (5x)
• replicons - Segment of DNA that replicates from a single origin of replication
• Prokaryotic replication - Single origin, single replicon
• DnaB: helicase
• DnaC: loading factor
• initiation

  A) DnaA

  B) 9-nuc repeat

  C) 13-nuc repeat

  
• initiation

  A) DnaBC

  B) DnaC

  C) pre-priming complex

  D) DnaB

  E) topoisomerase

  
• initiation

  A) SSBP

  B) DnaG

  C) Primosome

  D) replisome

  E) dna polymerase iii

  
• elongation and termination

  A) RMPs

  B) DNA polymerase I

  C) DNA Ligase

  
• Old Model - strands are synthesized separately
• trombone - new model
• ter element - defined termination sequence
• termination composed of 23 bp consensus sequence
• binding site of ter protein has contra-helicase activity