Effect of Temperature and pH on Enzymes

Cards (6)

  • Enzyme
    Speeds up chemical reactions by having a groove on their surface called the active site
  • Substrate
    The molecule that the enzyme reacts with, fits perfectly into the active site
  • Lock and key theory
    The substrate must fit perfectly into the active site
  • Effect of temperature on enzyme activity
    1. Gradually increase temperature
    2. Measure enzyme activity (rate of reaction)
    3. Activity increases as temperature increases
    4. Reach optimum temperature
    5. Activity rapidly decreases past optimum temperature
  • Optimum temperature

    The temperature at which the enzyme is working at the fastest possible rate, maximum frequency of successful collisions between substrate and active site
  • Denaturation
    At high temperatures, the enzyme molecule vibrates and the shape of the active site changes, so the substrate no longer fits perfectly