Temp and pH on ezymes

• As we increase the temperature, the activity of the enzyme increases (the reaction gets faster)

  
• As the temperature increases, the enzyme and substrate are moving faster so there are more collisions per second between the substrate and the active site.
• At a certain temperature, the enzyme is working at the fastest possible rate. That's called the optimum temperature.
• At the optimum temp, there is a maximum frequency of successful collisions between the substrate and the active site.
• As we increase the temperature past the optimum, then the activity of the enzyme rapidly decreases to zero.
• At high temperatures, the enzyme molecule vibrates and the shape of the active site changes.
• After the active site changes shape, the substrate no longer fits perfectly into the active site. The active site is denatured.
• The enzyme has an optimum pH, where the activity is maximum.

  
• If we make the pH more acidic or more alkaline then the activity drops to zero.
• The active site denatures if the conditions are too acidic or too alkaline.