How do you dissect a plant to prepare a section of tissue?
Use scalpel to cut cross-section of stem. Cut sections as thinly as possible - better for microscopeviewing
Use tweezers to gently place cut sections in water until use them. = stops them drying out
How do you dissect a plamt steps3-4?
3. Transfer each section to a dish containing a stain (eg TBO) and leave for one minute. TBO stains lignin in walls of xylemvesselsblue-green. = lets you see position of xylemvessels +examinestructure
4. rinseoff sections in water and mount each one onto a slide
What is TBO?
toluidineblue
What does TBO do?
Stains the lignin of the walls of the xylem vessels blue-green
what can you use?
different stains to highlight the different parts of the cells
