What is the test for non-reducing sugars?
After the test for reducing sugars, you should:
-Add dilute hydrochloric acid and boil to the test sample (hydrolyse the polysaccharides/disaccharides into its constituent monosaccharides)
- Then add sodium hydrogencarbonate to neutralise the acid. (Benedict's reagent does not work in acidic conditions)
- Add the sample to Benedict's reagent and then heat the sample.
-If the solution turns brick red then a non-reducing sugar is present. If the solution stays blue then there isn't a non-reducing or reducing sugar present.