Investigating transpiration
1: Cut a shoot underwater to prevent air entering xylem then place in tube.
2: Set up apparatus, ensuring it is airtight then dry leaves of the shoot.
3: Remove capillary tube to allow a single air bubble to form then place tube back.
4: Set up environmental factor (airflow/humidity/light intensity/temperature) then leave plant to adapt to environment for 5 minutes.
5: Record bubble's starting location, leave for a set period of time then record ending location.
6: Change the intensity of the factor being tested, reset bubble by opening tap then repeat.