Can be used to separate blood proteins and to identify hemoglobin
Electrophoresis
1. An electric field is applied to a solution of protein molecules
2. Allows the molecules to migrate at a rate depending on its size or charge
PAGE
The samples are run on an inert matrix composed of cross-linked polyacrylamide
Sodium dodecyl sulfate (SDS)
An anionic detergent used to denature and to mask the intrinsic charge of the proteins so that they migrate solely on the bases of their molecular weight
Molecular weight marker
A solution of proteins of known molecular weight run together with the samples and used as a standard for determining the molecular weight of the sample
Denaturing PAGE
Plasma and red blood cell lysates will be analyzed
Oxygen is required by the cells to utilize aerobic pathways in the production of ATP
In large, multicellular animals, diffusion of oxygen from the environment into the organism is insufficient to supply metabolic needs
This has led to the development of complex oxygen exchange surfaces and organs which comprise the respiratory system
Hemoglobin
Found in the red blood cells, it picks up oxygen in the gills and lungs and transports it to the tissues
Hemoglobin molecule in vertebrates
Consists of four globin chains, each with an iron-containing heme group
Typically composed of two polypeptide chains and two polypeptide chains
Plasma portion of blood
Mostly water, containing several plasma proteins which function in defense, clotting and transport of molecules
