Remove the needle from the syringe and discard it in a sharps container.
Rationale:
The needle must be removed to attach the transfer device.
Attach the syringe hub to the transfer device hub, rotating it to ensure secure attachment.
Rationale:
Secure attachment is necessary to prevent blood leakage during transfer.
Hold the syringe vertically with the tip down and the transfer device at the bottom.
Rationale:
This ensures vertical placement of tubes to prevent additive carryover.
Place an ETS tube in the barrel of the transfer device and push it all the way to the end.
Rationale:
The device has an internal needle that will puncture the stopper and allow blood to flow into the tube.
Follow the order of draw if multiple tubes are to be filled.
Rationale:
The order of draw is designed to prevent additive
carryover between tubes.
Keep the tubes and transfer device vertical.
Rationale:
This ensures that tubes fill from bottom to top, preventing additive contact with the needle and cross-
contamination of subsequent tubes.
Let tubes fill using the vacuum draw of the tube. Do not push on the syringe plunger.
Rationale:
Forcing blood into a tube by pushing the plunger can hemolyze the specimen or cause the tube stopper to pop off, splashing tube contents.
If you must underfill a tube, hold back the plunger to stop blood flow before removing it.
Rationale:
Tubes quickly fill until the vacuum is gone. Holding back the plunger stops the tube from filling
Mix additive tubes as soon as they are removed.
Rationale:
Additive tubes must be mixed immediately for proper function, including preventing clot formation
in anticoagulant tubes.
When finished, discard the syringe and transfer device unit in a sharps container.
Rationale:
Removing the transfer device from the syringe would expose the user to blood in the hubs of both units. The transfer device must go into the sharps container because of its internal needle.