Gene Expression Techniques

• Techniques for Studying Transcription Regulation

  • G-less Cassette Transcription Assays
  • Genealogical Tracking
  • R-loop Analysis
  • Intracellular Localization Studies
• 1. less Cassette Transcription Assays

  Used to study promoter activity by analyzing the binding strength to cis-elements. This method involves transcribing a DNA segment lacking guanine (G) bases, which is then analyzed through SDS-PAGE to measure the transcription output.
• Genealogical Tracking

  Utilizes PCR and DNA sequencing to trace phenotypic traits and their genetic origins across generations.
• 1. loop Analysis

  Investigates the structures formed by RNA-DNA hybrids, which can influence transcription and genomic stability.