This video is for paper one of GCSE biology and combined science
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Microscopy
The study of small objects using a microscope
Light microscope
First developed in the mid 17th century
Uses light to form an image
Can be used to view live specimens
Relatively cheap and easy to use
Can magnify up to 2,000 times
Resolution
The ability to see two things as separate objects
Light microscope
Has a low resolution with a resolving power of around 200 nanometers
Electron microscope
Developed by scientists in the 1930s
Uses electrons to form an image
Specimens must be dead first
Very expensive and has many conditions
Can magnify up to 2 million times
Has a high resolution of 0.2 nanometers
The electron microscope is much better than the light microscope in terms of magnification and resolution
The high magnification and resolution of the electron microscope allows scientists to see and understand lots more about the subcellular structures of cells
Animal cell
Contains the following organelles: nucleus, cell membrane, cytoplasm, mitochondria, ribosomes
Nucleus
Controls the cell's activities and contains genetic material (DNA)
Cell membrane
Responsible for controlling the passage of substances in and out of the cell
Cytoplasm
Liquid gel that fills the entire cell and is where chemical reactions occur
Mitochondria
Where aerobic respiration occurs, releasing energy for the cell
Ribosomes
Site of protein synthesis
Plant cell
Contains the following organelles in addition to those in animal cells: chloroplasts, permanent vacuole, cell wall
Chloroplasts
Contain chlorophyll and are the site of photosynthesis
Permanent vacuole
Filled with cell sap, helps keep the cell rigid
Cell wall
Made of cellulose, strengthens and supports the plant cell
Animal and plant cells are both eukaryotic cells
Eukaryotic cells
Have a cell membrane, cytoplasm, and genetic material enclosed in a nucleus
Prokaryotic cells
Lack a true nucleus, have genetic material as a single loop of DNA in the cytoplasm, may have plasmids and a cell wall, but lack organelles like mitochondria and chloroplasts
Specialized animal cells
Sperm cell: has a tail for movement, acrosome to break down egg, large nucleus
Muscle cell: has many mitochondria for energy, special proteins for contraction
Nerve cell: has a long axon to carry electrical impulses, dendrites to connect to other cells, nerve endings to release chemical messengers
Specialized plant cells
Root hair cell: has a large surface area, large permanent vacuole, many mitochondria
Xylem cell: forms long hollow tubes, has spiral lignin to strengthen and support
Phloem cell: has sieve plates to allow movement of dissolved food, companion cells with mitochondria to provide energy
Diffusion
The spreading out of particles in a solution or gas from an area of higher concentration to an area of lower concentration
The diffusion of water molecules from a dilute solution to a more concentrated solution through a partially permeable membrane
Osmosis is important in animal cells to maintain the right internal solute concentrations
Left side of the membrane
Has more water molecules
Right side of the membrane
Has less water molecules and more sucrose or solute molecules, therefore it is the concentrated side
Osmosis
1. Occurs down a concentration gradient or from a dilute solution to a more concentrated solution
2. Net movement of water is from the left side to the right side
3. Water concentration will eventually be equal on both sides
4. Water molecules will continue to move back and forth across the partially permeable membrane but there's no further net movement of water, the water is balanced on both sides
Importance of osmosis in animal cells
Ensures solutes like glucose and salts are at the right concentration inside the cell
The internal environment needs to be kept just right for the cell to work
The difference in concentration between the cell's internal environment and the external solution determines how much osmosis occurs
What happens when a red blood cell is put into a hypotonic solution
Water moves into the cell, stretching it, and the cell may even burst if a lot of water moves in, killing the cell
What happens when a red blood cell is put into an isotonic solution
Nothing happens, as the two solutions are the same concentration, so there is no net movement of water (no osmosis)
What happens when a red blood cell is put into a hypertonic solution
Water moves out of the red blood cell and into the beaker, as the red blood cell solution is more dilute, the cell will shrink and not function properly
Required practical A by osmosis looks at the effect of concentration of salt or sugar on the mass of plant tissue
Active transport
Substances move from a low concentration to a high concentration, against the concentration gradient, requiring energy from respiration