effect of ph on amylase

Cards (5)

    • test tubes
    • test tube rack
    • water baths ( bunsen burners and beakers)
    • spotting tiles
    • 5cm^3 measuring cylinder
    • glass rods
    • a stop watch
    • starch solution
    • amalyse solution
    • iodine solution
    • thermometers
    equipment needed
  • step 1

    place one drop of iodine somution into each depression on the spotting tile
  • Step 2

    set up water baths for every temperature you want to test (suggest one cold with ice,one at room temperatur, one around body temperature 35-40 °C and one above 50 degrees
  • step 3

    measure out 5cm^3 of star h solution,using the measuring cylinder or syringe, into 4 different test tubes
  • step 4

    place one test tube of starch solution into each water bath