Antigen detection process
1. Container coated with monoclonal antibodies specific for the antigen being screened for
2. Sample to be tested (e.g. blood/urine) is added to the container
3. If the specific antigen is present it will bind to the antibodies
4. A second monoclonal antibody is added that binds to another site on the antigens
5. The second Ab has an enzyme attached
6. The sample is then washed
7. The substrate for the enzyme is then added
8. The substrate is hydrolysed by the enzyme on the second antibody
9. This produces a colour change, indicating a positive result (ie the antigen was present in the original sample)