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Learning Objectives
assays
assays - creatinine
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Created by
Jayna Ramesh
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Cards (4)
Jaffe-kinetic method of creatinine analysis creatinine combined with
sodium picrate
in
alkaline
solution yields
red orange
color
creatininase-CK
A)
creatinine
B)
H2O
C)
creatininase
D)
creatine
E)
creatine
F)
ATP
G)
CK
H)
creatine phosphate
I)
ADP
J)
ADP
K)
phosphoenolpyruvate
L)
PK
M)
pyruvate
N)
ATP
O)
pyruvate
P)
NADH
Q)
H+
R)
LD
S)
lactate
T)
NAD+
20
creatininase-CK
Change in absorbance is measured bc of
oxidation
of
NADH
Requires
large
sample
creatininase - H2O2
A)
creatinine
B)
H2O
C)
creatininase
D)
creatine
E)
creatine
F)
H2O
G)
creatinase
H)
sarcosine
I)
urea
J)
sarcosine
K)
O2
L)
H2O
M)
sarcosine oxidase
N)
glycine
O)
CH2O
P)
H2O2
Q)
H2O2
R)
colorless substrate
S)
peroxidase
T)
colored product
U)
H2O
21