amylase-test

• Investigating the effect of pH on enzyme activity

  1. Place single drops of iodine solution in rows on the tile

  2. Label a test tube with the pH to be tested

  3. Use the syringe to place 2cm3 of amylase in the test tube

  4. Add 1cm3 of buffer solution to the test tube using a syringe

  5. Use another test tube to add 2cm3 of starch solution to the amylase and buffer solution, start the stopwatch whilst mixing using a pipette

  6. After 10 seconds, use a pipette to place one drop of the mixture on the first drop of iodine, which should turn blue-black

  7. Wait another 10 seconds and place another drop of the mixture on the second drop of iodine

  8. Repeat every 10 seconds until iodine solution remains orange-brown

  9. Repeat experiment at different pH values – the less time the iodine solution takes to remain orange-brown, the quicker all the starch has been digested and so the better the enzyme works at that pH
• Investigating the effect of pH on enzyme activity
• Enzymes are folded into complex 3D shapes that allow smaller molecules to fit into them. The place where these molecules fit is called the active site close active siteThe part of the enzyme to which a specific substrate can attach or fit on to..
• How does pH affect enzyme function? When the pH is above the optimal level, the enzyme is denatured. Denaturation means that the shape of the enzyme changes making it unable to bind with the active site of the substrate.