Bio Topic 1 (Paper 1)

    Cards (56)

    • Eukaryotic cells
      Animal and plant cells
    • Prokaryotic cells
      Bacterial cells
    • Structures in eukaryotic cells
      • Cell membrane
      • Cytoplasm
      • Nucleus containing DNA
    • Structures in prokaryotic cells
      • Cell wall
      • Cell membrane
      • Cytoplasm
      • Single circular strand of DNA and plasmids
    • Organelles
      Structures in a cell that have different functions
    • Organelles in animal and plant cells
      • Nucleus
      • Cytoplasm
      • Cell membrane
      • Mitochondria
      • Ribosomes
    • Organelles only in plant cells
      • Chloroplasts
      • Permanent vacuole
      • Cell wall
    • Structures in bacterial cells

      • Cytoplasm
      • Cell membrane
      • Cell wall
      • Chromosomal DNA (circular)
      • Plasmids
      • Flagella
    • Differentiation
      A process that involves the cell gaining new sub-cellular structures in order for it to be suited to its role
    • Specialised animal cells
      • Sperm cells
      • Egg cells
      • Ciliated epithelial cells
    • Specialised plant cells
      • Root hair cells
      • Xylem cells
      • Phloem cells
    • Light microscope
      Has two lenses, usually illuminated from underneath, maximum magnification of 2000x and resolving power of 200nm
    • Electron microscope
      Uses electrons instead of light, can achieve magnification up to 2,000,000x and resolving power of 10nm (SEM) and 0.2nm (TEM)
    • Electron microscopes have allowed the discovery of viruses and detailed examination of proteins
    • Magnification
      Magnification of the eyepiece lens x magnification of the objective lens
    • Size of an object
      Size of image/magnification = size of object
    • Standard form
      Multiplying a number by a power of 10 to get bigger or smaller, with the 'number' between 1 and 10
    • Orders of magnitude
      Understanding how much bigger or smaller one object is compared to another
    • Prefixes
      Go before units of measurement to show the multiple of the unit
    • Estimation
      Useful when we don't know the exact number of something, by taking a sample and extrapolating
    • Parts of a light microscope
      • Eyepiece
      • Barrel
      • Turret
      • Lens
      • Stage
    • Using a light microscope
      1. Place slide on stage
      2. Look through eyepiece
      3. Turn focus wheel
      4. Start with lowest magnification, then increase
    • Preparing a slide
      1. Take thin layer of cells
      2. Add chemical stain
      3. Apply cells to slide
      4. Lower coverslip
    • Enzymes
      Biological catalysts that increase the rate of reaction without being consumed
    • Active site
      The uniquely shaped site on an enzyme where the substrate binds
    • Lock and key hypothesis
      The shape of the substrate is complementary to the active site, forming an enzyme-substrate complex
    • Ion
      Measured size / Actual size
    • Actual size
      Measured size / Magnification
    • Total magnification
      Objective lens magnification x Eyepiece lens magnification
    • Enzymes are biological catalysts (a substance that increases the rate of reaction without being used up)
    • Enzymes
      • They are present in many reactions - allowing them to be controlled
      • They can both break up large molecules and join small ones
      • They are protein molecules and the shape of the enzyme is vital to its function
      • Each enzyme has its own uniquely shaped active site where the substrate binds
    • Lock and Key Hypothesis (how enzymes work)
      1. The shape of the substrate is complementary to the shape of the active site (matches the shape of the active site), so when they bond it forms an enzyme-substrate complex
      2. Once bound, the reaction takes place and the products are released from the surface of the enzyme
    • Enzyme specificity
      Enzymes can only catalyse reactions when they bind to a substrate that has a complementary shape, as this is the only way that the substrate will fit into the active site
    • Enzymes
      • They require an optimum pH and temperature, and an optimum substrate concentration
    • Optimum temperature for enzymes in humans
      A range around 37 degrees Celsius (body temperature)
    • As temperature increases
      The rate of reaction increases up to the optimum, but above this temperature it rapidly decreases and eventually the reaction stops
    • Denaturation
      When the bonds that hold the enzyme together break, changing the shape of the active site so the substrate can no longer 'fit into' the enzyme
    • Optimum pH for most enzymes
      pH 7 (neutral)
    • If the pH is too high or too low

      The forces that hold the amino acid chains that make up the protein will be affected, changing the shape of the active site so the substrate can no longer fit in
    • As substrate concentration increases
      The rate of reaction will increase up to a saturation point, after which increasing the substrate concentration will have no effect
    See similar decks