Proteins

• What does biuret’s test is for

  Presence of peptide bonds
• What are the reagents used for biuret’s test

  sodium hydroxide and copper sulfate
• Biuret's test

  Test used to detect peptide bonds
• Positive result in Biuret's test


  Violet/Purple color
• Millon's test


  Test that detects Tyrosine
• Reagents in Millon's test

  Nitric Acid and Mercuric nitrate
• Positive result in Millon's test

  Brick red precipitate
• Xantoproteic test


  Test that detects Aromatic Rings (Phenylalanine and Tyrosine)
• Reagents in Xantoproteic test

  Nitric Acid and Ammonium Hydroxide
• Positive result in Xantoproteic test

  Yellow or Orange
• Hopkin's-Cole test


  Test that detects Tryptophan
• Reagents in Hopkin's-Cole test

  Glyoxylic Acid and Sulfuric Acid
• Positive result in Hopkin's-Cole test


  Violet ring
• Test for sulfur


  Test that detects free sulfur bonds in amino acids like cysteine and cystine
• Reagents in Test for sulfur

  Sodium Hydroxide and Lead (II) Acetate
• Positive result in Test for sulfur


  Black Precipitate
• What is the objective of the Ninhydrin test?

  To detect free alpha amino groups.
• What reagents are used in the Ninhydrin test?

  Ninhydrin Reagent.
• What color indicates a positive result in the Ninhydrin test?

  Purple or Blue
• What is the objective of the Alcohol test?

  To destroy hydrogen bonds
• What reagents are used in the Alcohol test?

  95% and 70% alcohol.
• What indicates a positive result in the Alcohol test?

  Cloudy white precipitate.
• What is the objective of the Heat test?

  To destroy hydrogen bonds.
• What reagents are used in the Heat test?

  Sodium Hydroxide and Copper Sulfate.
• What color indicates a positive result in the Heat test?

  Violet/Purple color.
• What is the objective of the Ninhydrin test?

  To detect free alpha amino groups.
• What reagents are used in the Ninhydrin test?


  Ninhydrin Reagent.
• What color indicates a positive result in the Ninhydrin test?

  Purple or Blue.
• What is the objective of the Alcohol test?

  To destroy hydrogen bonds.
• What indicates a positive result in the Alcohol test?

  Cloudy white precipitate.
• What is the objective of the Heat test?

  To destroy hydrogen bonds.
• What reagents are used in the Heat test?

  Sodium Hydroxide and Copper Sulfate.
• What color indicates a positive result in the Heat test?

  Violet/Purple color.
• What is the objective of the Ninhydrin test?

  To detect free alpha amino groups.
• What reagents are used in the Ninhydrin test?

  Ninhydrin Reagent.
• What is the purpose of Heller’s test

  proteins
• what is the positive result of heller’s test

  white percipitate
• what reagent is used in heller’s test

  nitric acid