related study q's - iron and tibc

• Ferritin consists of protein shell surrounding an iron core
• Ionic iron needs to be shielded from fluids of body as it can cause oxidative damage
• Apotransferrin is the protein that transports iron around the body
• Describing prodedure for total iron determination

  §  Release iron from transferrin, first decreased pH

  §  Iron reduced from 3+ to 2+ state with hydroxylamine

  §  Iron is complexed with chromagen (e.g. ferrozine)

  §  Colored complex forms

  §  Absorbance is read at high absorbance (560 nm in our lab)

  §  Follows Beer’s law