1. Write down a hypothesis of the effect of a change in an abiotic factor (eg. light intensity) on the distribution of the plant species
2. Lay down a tape measure from the base of a tree to an open area of ground/ along a location with an ecological gradient
3. Place the quadrat along the '0' end of the tape measure, with one corner touching the '0' mark
4. Count the number of plants and record it in a table
5. Place the quadrat 5 m up the tape measure and repeat step 3
6. Repeat step 4 at 5 m intervals until you reach the end of the transect line
7. Gather data from your class to find the mean number of plants at each point along the transect
8. Plot a graph of 'number of plants' against the ecological gradient that is observed as the distance along the transect line increases. Compare your results to your hypothesis