topic1: good lab practice & general lab safety

• what is Good Laboratory Practice (GLP)?

  A quality control system covering the organizational process and the conditions under which non-clinical health and environmental safety studies are planned, performed, monitored, recorded, reported and archived
• Importance of GLP

  • It promotes the quality and validity of test data
  • It promotes mutual recognition of study data internationally
  • It reduces duplicative testing and thus shortens the time to market
• does GLP apply to the analysis of samples from Human Clinical Trials?

  
  

  no, that's Good Clinical Practice (GCP)
• general properties of GLP: Organization and Personnel

  • ensure that the necessary facilities, materials, equipment, procedures and resources are in place thruought
  • Staff are trained in relevant procedures and are responsible for the quality of their data
• general properties of GLP: Apparatus, Materials and Reagents

  • All apparatus, should be fit for purpose
  • Chemicals, reagents and solutions should be clearly labelled and fit for purpose
• general properties of GLP: Test Systems

  • Test System is the biological, chemical or physical system used to conduct a study
  • Test system should be appropriate and well maintained to ensure the quality of data obtained
  • biological test system should be characterized appropriately and suitably maintained for quality data
• what are Reference Itemsused for?

  • Reference Items are used for comparative purposes, or to validate a test
• what is Standard Operating Procedures

  • documented procedures which describe how to perform tests or activities
  • help ensure the quality and integrity of data
• where is raw data stored?

  • all raw data is transferred for safe keeping to a designated Archive
  • Access to the Archive is carefully controlled to protect the contents
  • Materials may be kept for many years
• 5S System for Good Housekeeping

  • It provides workers and supervisors with an understanding on how quality and productivity can be improved through good systematic housekeeping
• The 5S System for Good Housekeeping

  • Seiri (Sort) - Remove all unnecessary items from the workplace
  • Seiton (Set in order) - Create a specific location for everything that remains
  • Seiso (Shine) - Clean and inspect the workplace
  • Seiketsu (Standardize) - Standardize best practices
  • Shitsuke (Sustain) - Make 5S part of daily work culture. Audit regularly to maintain standards
• Seiri (Sort)

  Sort and throw away unnecessary items. Emphasis on organization.
• Seiton (Set in order)

  Ensure there is place for everything and everything is in place. Emphasis on orderliness.
• Seiso (Shine)

  Cleaning and inspection at the same time. Emphasis on cleanliness.
• Seiketsu (Standardize)

  Make the rules and follow them. Emphasis on neatness, health and hygiene.
• Shitsuke (Sustain)


  Learning the virtue of good housekeeping. Emphasis on good conduct and discipline.
• Benefits of implementing 5S system for employees

  • Better co-operation between workers
  • Cleaner and more organized workplaces
  • More space are created for other things by discarding of old and unwanted material
  • Awareness of environmental issues
  • Less stress and cosier place to work
  • Better discipline
  • Easier to locate objects (files, tools, etc.)
  • Less chances of accidents occurring
• Benefits of implementing 5S system for employers

  • Better workers' productivity and efficient use of the time
  • Good usage of equipment and material
  • Less wastage of material
  • Reduction of costs
  • Better workers' moral
• Basic Laboratory Safety Rules and Practices - ATTIRE

  • Wear the correct laboratory coat at all times in the laboratory
  • Always wear closed and flat shoes and long pants when working in the laboratory. Sandals and tights are NOT ALLOWED!
  • Restrain long hair and loose clothing. Long fringe should be pinned up
  • Wear additional Personal Protection Equipment (PPE) as instructed
• Basic Laboratory Safety Rules and Practices - ENTERING THE LAB

  • Upon entering the Laboratory, locate First Aid cabinets, eyewash, emergency shower, fire extinguishers, emergency exits, broken glass containers and clean up materials for spills
• Basic Laboratory Safety Rules and Practices - LAB INCIDENT HANDLING

  • Report to teacher-in-charge if a laboratory accident has occurred
  • If you get in contact with chemicals in your eyes, wash your eyes immediately with large quantity of water and inform the teacher-in-charge
  • Report all breakages of glassware or damage to equipment, to the teacher-in-charge
  • Hand protection should be used when picking up broken glass. Broken glassware should be disposed of in a special container called "BROKEN GLASS"
• Chemical fume hood


  Designed to keep toxic or irritating fumes away from general lab area, Serves as a shield between worker and equipment, Can be used to handle odorous materials, toxic gases, reactive materials, chemicals that can spatter, aerosols, carcinogens, flammables or other toxic or volatile materials
• Chemical fume hoods

  • Fume hoods are designed to provide user protection from toxic or volatile chemicals by continuously delivering airflow away from the operator to the work area, Offers no protection to the product or the environment

  
• Biosafety Cabinets (BSC)

  Enclosed workspaces with a ventilated hood that is designed to contain pathogenic microorganisms during microbiological processes, Provides a safe environment for research involving infectious microorganisms or other hazardous particulates, Used in life science research, cell culture processing, etc.

  
• Biosafety Cabinets

  • Equipped with high-efficiency particulate air (HEPA) filters which effectively traps all known infectious agents and ensure that only microbe-free exhaust air is discharged from the cabinet, Can provide protection for user, product and environment

  
• Types of Biosafety Cabinets

  • Class I
  • Class II
  • Class III

  
• Importance of safety practices in pharmaceutical &biomedical industries

  • Handling of biological agents which may contain pathogens; work-related exposure to biological agents may be associated with several health problems, including infectious diseases, cancer and allergies
  • Needlestick and sharps injuries are one of the major risk factors for blood-borne infections
  • Handling of chemicals, e.g. cytotoxic
• Importance of safety practices in chemical industries

  • Chemical industries typically involve the use of hazardous chemicals, which can be toxic, flammable, or explosive. These chemicals, if not handled properly, may cause serious accidents (e.g. fire, explosion), leading to injury and even death of people, as well as damage and loss to the properties.
• Importance of safety practices in food industries

  • Slips, trips and falls
  • Scalding and burns by hot liquids or steam
  • Handling of sharp objects/ knives
  • Injury due to improper handling of the machinery
• Importance of safety practices in veterinary industries

  • Cuts and infection
  • Bites and scratches
  • Zoonotic disease