Microscopes

• A stain is iodine attached to cells to create contrast
• fluorescence microscopy is used to gain information about molecules and locate them.
• fluorescence microscopy uses fluorescent substance that are attached to molecules to locate it by shining ultraviolet light on the substance.
• Confocal technology provides a thin section of the specimen with high resolution
• Confocal technology works by having a laser concentrated on a speciman, the reflection of the laser is then converted into images.
• Transmission Electron Microscopy uses electron beams to produce images
• Scanning Electron MIcroscopy uses electrons that bounce off the object to produce an image
• Xray Crystallography is used to determine atomic and molecular structure
• Xray Crystallography works by a beam of xray passing through a crystal then diffracts to many directions
• Eyepiece enhances magnification and focus
• Coarse Adjustment brings specimens into view by moving the stage, is used before fine-adjustment
• fine adjustment achieves detailed image of specimen
• Revolving nosepiece can be rotated to select a different objective lense
• objective lenses has multiple lenses that varies magnification, can increase magnification
• stage provides support to hold the specimen
• stage clips secure the specimen in place
• diaphragm regulates the amount of light passing through the specimen, enhancing contrast and brightness
• lamp, source for light, makes specimen visible
• arm provides support and carrying handle, can be raised or lowered
• base is the foundation of microscope, prevents it from tipping over.
• Transmission electron, specimen is embedded in plastic
• scanning electron examines surface of specimen
• Confocal provides 3D images of specimen
• Transmission Electron has the highest magnification, (2 million mag)