Investigating amylase enzyme activity
1. Add drop of iodine solution to each reaction tube
2. Heat water to 26°C over Bunsen burner
3. Add 1cm3 amylase solution and 1cm3 buffer solution to boiling tube
4. Add 5cm3 starch solution
5. Mix immediately and start stopwatch
6. Record time taken for starch to be broken down
7. Repeat with buffer solutions of different pH values