Using a light microscope

Cards (3)

  • Equipment
    • Light microscope
    • Microscope slide
    • Cover slip
    • Onion
    • Iodine solution
    • White tile
    • Scalpel
    • Any other prepared plant and animal cell slides
  • Method
    1. Peel off an epidermal layer on the onion using forceps
    2. Put onto the microscope slide with a drop of water
    3. Add 2 drops of iodine solution to stain the cells
    4. Place the cover slip on by first placing one edge edge down horizontally, make sure there are no bubbles
    5. Place the slide on the stage of the microscope
    6. Set up the microscope
    7. Look into the eyepiece and turn the coarse adjustment knob to move the stage away until the image comes into focus
    8. Turn the nosepiece to select a high power objective
    9. Look into the eyepiece and turn the fine adjustment knob until the image comes into focus
    10. Write down the magnification
    11. Repeat these steps using a prepared slide
  • Wear safety goggles when handling the iodine solution