apparatus

• Borrowing and Returning of Apparatus and Equipment

  1. Fill up borrowers' slip issued by the Office of UNP Laboratory Services

  2. Borrow and reserve apparatus needed one day before the laboratory period

  3. Receive apparatus during the scheduled time of your laboratory

  4. Receive only clean and dry apparatus

  5. Check if all chemicals requested and apparatus were issued

  6. Report any breakage or damage to the laboratory staff

  7. Return only clean and dry apparatus and equipment

  8. No apparatus and equipment should be taken out from the premises of the UNP
• Breakage should be replaced or equivalent cost shall be paid at the cashier's office. Show receipt to the lab staff for recording and clearance of your breakage. Wait for the Official Receipt to be recorded.
• General Rules and Practices for Microbiology Laboratory Safety

  • Treat all microorganisms as potential pathogens
  • Be respectful of one another and the materials used in the microbiology lab
  • Wear a laboratory gown or other protective clothing
  • Sterilize equipment and materials
  • Disinfect work areas before and after use
  • Wash your hands
  • Never pipette by mouth
  • Do not smoke, eat or drink in the lab, nor store food in areas where microorganisms are stored
  • Label everything clearly
  • Always handle test tubes by the tube, not the top
  • Autoclave or disinfect all waste material
  • Discard all used materials into proper containers
  • Discard broken glass into the appropriate Broken Glass container
  • Clean up spills with care
  • All materials, cultures, and equipment must remain in the lab at all times
  • Refer to your instructor in case of doubt and emergency
  • Conserve water and energy
  • Photography, videos, and recordings can only be taken with instructor consent
• Failure to follow these safety rules may result in loss of points from the related activities. Violation of the rules may result in a referral to the Office of Student Affairs for disciplinary action.
• The effects on human pregnancy of chemical agents used in this course are unknown, and a pregnant woman is advised to consult her physician before taking this course
• Safety data sheets are available upon request. As contact with dyes used in staining may have potential teratogenic effects, a pregnant woman should avoid staining procedures as directed by her physician.
• A student who has a compromised immune system or has had a recent extended illness should talk with the instructor before working in the microbiology laboratory.
• In the unlikely event that we must leave the classroom, please reassemble in the vacant area in front of the Science building
• Apparatus and Equipment Used in a Microbial Laboratory

  • Plain Test Tube
  • Screw-capped Tube
  • Centrifuge Tube
  • Fermentation Tubes
  • Durham Fermentation Tubes
  • Glass slides
  • Depression slides
  • Cover slip
  • Watch Glass
  • Erlenmeyer flasks
  • Beaker
  • Pipette
  • Funnel
  • Graduated cylinder
  • Aspirator
  • Inoculating Loop
  • Inoculating Needle
  • Test tube rack
  • Tripod
  • Wash bottle
  • Alcohol lamp
  • Laboratory oven/incubator
  • Autoclave
  • Centrifuge
  • Hot plate
  • Microscope
  • Wire gauze
  • Test tube brush
  • Evaporating dish
  • Volumetric flask
  • Candle jar
  • Stirring rod
  • Spatula
  • Clay triangle
  • Test tube holder
  • Triple beam balance
  • Crucible with cover
• Plain Test Tube

  Used to hold, mix, and heat chemical experiments. Used as homes for microorganisms when people want to culture (grow) them
• Screw-capped Tube

  Used to grow, culture, and identify bacteria in a microbiology laboratory
• Centrifuge Tube

  Used to separate a substance into its components
• Fermentation Tubes

  Used to verify and measure gas production in fermentation experiments
• Durham Fermentation Tubes

  Used in microbiology to detect production of gas by microorganisms
• Glass slides

  Used to hold objects or specimen for examination under a microscope
• Depression slides

  Used to examine living microorganisms in a hanging drop
• Cover slip

  Keep solid specimens pressed flat, and liquid samples into a flat layer of even thickness
• Watch Glass

  Used to hold solids during weighing and cover beakers during sample preparation
• Erlenmeyer flasks

  Used for the preparation of microbial cultures
• Beaker


  Used for reaction container or to hold liquid or solid samples
• Pipette


  Used to measure a volume of solution extremely precisely
• Funnel


  Used for filtering, filling, decanting or transferring liquids or powder from vessels to another
• Graduated cylinder


  Used to measure the volume of a liquids, chemicals and solution. Are more accurate and precise than laboratory beaker
• Aspirator


  It serves as a vacuum source for filling reagents through a pipette and also help control the flow of liquid from the dropping bottle
• Inoculating Loop


  Used for transfer and streaking of microorganisms onto solid culture media
• Inoculating Needle


  Used for transfer and streaking of microorganisms onto solid culture media
• Test tube rack


  Used to hold upright multiple test tubes at the same time
• Tripod


  Used to support or hold the flasks and beakers during experiments
• Wash bottle


  Used to rinse various pieces of laboratory glassware, such as test tubes and round bottom flasks
• Alcohol lamp


  Used for heating, sterilization, and combustion in a laboratory
• Laboratory oven/incubator


  Used to grow and maintain microbiological cultures or cell culture
• Autoclave


  Used to decontaminate certain biological waste and sterilize media, instruments and lab ware
• Centrifuge


  Used to separate particles or macromolecules like cells, sub-cellular components, proteins, and nucleic acid
• Hot plate


  Used to heat substances in containers such as beaker, flasks or test tubes
• Microscope


  Used to observe small objects, even cells
• Wire gauze


  Used to provide a stable, heat-resistant surface for containers such as flasks, beakers, and test tubes, and helps to distribute heat evenly
• Test tube brush


  Used to clean test tubes and narrow mouth laboratory glassware's
• Evaporating dish


  Used to evaporate excess solvents-most commonly water-to produce a concentrated solution or a solid precipitate of the dissolved substance
• Volumetric flask


  Used for precise dilutions and preparation of standard solutions
• Candle jar


  Used to grow bacteria requiring an increased CO2 concentration