Enzymes

• 1)Label each test tube (0C,20,C)
• 2)Pour equal amounts of milk in each test tube
• 3)Take the 20C test tube, that's been filled with milk, and add 5 drops of phenolphthalein solution and stir with a glass rod

  The phenolphthalein solution will visually indicate the change in pH
• 4) Place the 20C test tube into a water bath, using a thermometer wait for it to heat up to 20C.
• 5)Using a pipette, measure 1mL of lipase solution and release it into the tube, then immediately start the stop watch

  Using a glass rod, stir the contents in the tube to evenly distribute the lipase solution
• 6)When the solution becomes milky white, stop the stop watch and record the time.
• Independent variable: Temperature of the water

  Dependant variable: Time taken

  Control: Amount of lipase solution, pheno indicator
• Hazard: The pheno indicator is flammable and and irritant to skin

  -> Wear gloves, keep away from open flames
• Enzymes are catalysts that speed up chemical reactions.
• Enzymes have active sites which are essentially key holes that fit into certain molecules they are working on. When the right molecules comes along it fits into the active site so chemical reactions are faster