6. Microbiology

• Method A

  1. Glass petri dishes and agar gel must be sterilised before use in a pre-sterilised plastic petri dish
  2. Pour the sterile agar plates and allow to set fully
  3. Sterilise the inoculating loop, by heating it in the Bunsen burner flame
  4. Dip the inoculating loop into the microorganism solution and make streaks on the surface of the agar plate
  5. Replace the lid as soon as possible, secure with tape, store upside down
  6. Incubate at a maximum temperature of 25 degrees
• Method B

  1. Soak the filter paper disks in a variety of solutions, use either different concentrations of the same solution, or a variety of different solutions
  2. Pour the sterile agar plates and allow to set fully
  3. Measure the clear area around the filter paper disks. A control disk must be includes