CO2 is taken up at night through the open stomata, Converted into malate by phosphoenolpyruvate carboxylase (PEPC), and stored in the vacuole, During the day, CO2 is produced by a decarboxylation reaction and used by RuBisCO, By utilizing the CO2 stored in the vacuole, stomata can be kept closed during the day to reduce water loss by transpiration