H+ ions actively pumped out of companion cell using energy from ATP.
High conc of H+ ions causes facilitated diffusion back into companion cell. Sucrose carried with H+ ions through cotransport proteins in plasma membrane
increasing conc of sucrose in companion cell causes it to diffuse through plasmodesmata into sieve tube element
phloem loading
-different routes
symplast
apoplast
vacuolar
sucrose moved against conc. Gradient from cells where its made or being stored - why its an active process (need ATP)
ATP used to create conc. gradient for H+ ions pumped out of companion cells - increases conc outside cells
translocation: transport of assimilates throughout a plant
source: parts of a plant which load assimilates such as sugars, into phloem sieve tubes
sinks: parts of the plant which remove assimilates from phloem sieve tubes
pressure flow hypothesis: most widely accepted explanation of sap movement in plants
hydrophytes: plant adapted to living in water or where its very wet.
keep leaves afloat so they're in the air and absorb light
large air spaces in leaves to keep them afloat
flexible stems and leaves to reduce damage by water current
cacti (xerophyte)
store water in stem which is ribbed or fluted so it can expand
leaves reduced to spines to reduce surface area (limits water loss)
can trap moisture (reduces wp gradient)
stem is green can photosynthesise
roots are widespread or deep
close stomata at hottest time of day
xerophytes: plants that are adapted to living in very dry conditions
can close stomata when water availibility is low
some plants maintain a high salt conc in leaves to reduce wp
have long roots that can reach water deep underground
cohesion tension theory
water is a polar molecule - positive and negative charges not evenly distributed
in xylem, water molecules arrange so that positive and negative charged poles lie next to eachother - causes molecules to cohere
if some leave by transpiration others are pulled behind them
xylem tissue made of vessels to transport water and mineral ions and fibres (provide support for plant)
living cells called parenchyma cells - act as packing tissue to separate and support vessels
ligning forms patterns in cell walls: spiral, annular (weakest, most flexible), reticulate (strongest, least flexible)
xylem able to stretch as plant grows due to lignin being deposited in different patterns
places where lignification is not complete gaps called bordered pits form
tracheids
transport water, mineral salt
not perforated at end
found in all vascular plants
ends overlap
help provide some support
xylem: big tubes surrounded by tracheids
medulla
makes up 'bulk' of root
cortex
found outside epidermis. provides strength and can also photosynthesise if chloroplasts are present
pericycles
just inside endodermis and contains meristem cells which retain the ability yo divide
endodermis
special sheath/ covering of cells that play a role in getting water to xylem vessels
monocots
parallel lines in leaves
floral part in threes
one cotyledon
vascular bundles throughout
pollen grain has one pore
dicots
vein like pattern in leaves
floral parts in fours or fives
two cotyledons
vascular bundles arranged in rings
pollen grains have 3 pores
cotyledon
and embyronic leaf in seed-bearing plants, one or more of which are the first leaves
dicotyledonous
collenchyma and sclerenchyma may also be found to produce strength and support - vascular bundles found in centre of roots
companion cells
small cells found inbetween sieve tube elements
have a large nucleus and dense cytoplasm
produce ATP needed for active processes
responsible for carrying out metabolic processes needed to load sucrose into sieve tube elements
parenchyma
packing cells
phloem
transport assimilates and made of sieve tubes and companion cells
sieve tube elements
elongated cells - line up to form sieve tubes
lack a nucleus and have little cytoplasm - depends on companion cells for providing proteins and ATP
sieve plates are perforated (have pores)
have thin cell walls - allows cell sap to move easily from one cell to the next
transpiration: loss of water vapour from aerial parts of plant. - limited by waxy cuticle
water enters leaf through xylem and moves into spongy mesophyll cells by osmosis and passes along cells by apoplast pathway
water evaporates from cell walls of spongy mesophyll cells
water is lost from leaf through stomata. Relies on there being a water vapour potential gradient
schlerenchyma fibres
allow fluids/ nutrients to move through small lumen back and fourth
provide strength and support to plant
mature cells are dead
parenchyma cells
living cells, intracellular shape
in roots, can store starch
in leaves can have chloroplasts called chlorenchyma
chlorenchyma
living cells, little/ no intracellular shape - provide support with some flexibility
water inside cell exerts a pressure on the cell wall - pressure potential - as pressure potential builds up, reduces the influx of water
cells placed in solutions with negative wp, water lost from inside cell
flaccid=plasmolysed
reasons for transpiration
transports mineral ions up plant
maintains cell turgidity
photosynthesis
water uptake - solutions moves to where its more negative
vacuolar pathway
enters the cell cytoplasm through plasma membrane ans is able to enter and pass through the vacuoles. water moves by osmosis
apoplast pathway
passes through spaces in cell walls and between cells. does not enter cells and therefore moves by mass flow (not osmosis)
symplast pathway
water enters cytoplasm through plasma membrane and moves through plasmodesmata, from one cell to next. Water moves by osmosis
transpiration stream
movement of water from soils, through plant, to air surrounding leaves
main force driving this is water potential gradient
root pressure
minerals pumped into medulla and xylem which draws water into medulla by osmosis. pressure in root medulla builds up and pushes water up xylem.
transpirational pull
water molecules attracted to each other (cohesion). As water lost from leaves, whole column of water pulled upwards
-pull from above creates tension in column of water. This is why xylem vessels are strengthened with lignin or they'd collapse- cohesion tension theory.
casparian strip
water and minerals enter root hair cells by osmosis
water moves down wp gradient from root cortex to endodermis of vascular bundle
water moves via apoplast and symplast pathways
when water reaches endodermis, it is blocked by casparian strip which contains waxy material (suberin) through which water cannot pass