The polymerase chain reaction (PCR) is a technique for the amplification of DNA
In PCR, primers are complementary to specific target sequences at the two ends of the region of DNA
PCR has lots of applications including to help solve crimes and diagnose genetic disorders.
Stage 1 DNA is heated to 92–98 ⁰C
To separate the DNA strands.
Stage 2 DNA is cooled to 50-65 ⁰C
To allow complementary primers to bind
Stage 3 DNA is heated to between 70-80 ⁰C
To allow heat-tolerant DNA polymerase to replicate the DNA
Repeated cycles of heating and cooling amplify this region of DNA.
