Practical procedure to investigate the effect of pH on enzyme activity
1. Place a tripod and gauze on a Bunsen burner
2. Heat water in beaker until 35°C (use thermometer)
3. Get spotting tile and fill spots with iodine solution
4. Using syringe add 1cm³ of amylase and 1cm³ of buffer solution (pH 5) to test tube
5. After five minutes add 5cm³ of starch solution to test tube
6. Start time and every 30s using a pipette add some solution to spot on spotting tile
7. Do this until the solution turns orange/red from blue/black and stop timer
8. Repeat with different pHs of buffer solution and compare timings