Firstly place 1 drop of iodine in each spotting well, then set up 3 test tubes with 2cm of starch, 2cm of amylase and 2cm of pH 5 buffer. Then place them in a water bath for 10 mins and mix solutions into 1 test tube. After that place them back in water bath and start a stopwatch whereby every 30secs remove 1 drop of it and put in spotting well and continue until solution turns orange.