to prepare an uncontaminated culture:
• Petri dish and agar sterilised before use
• to kill unwanted bacteria
• inoculating loop passed through flame / sterile swab• to sterilise / kill (other) bacteriaInoculation
• loop/swab used to spread/streak bacterium onto agar• lid of Petri dish opened as little as possible
• to prevent microbes from air entering
• to prevent microbes from air entering• incubate
• to prevent microbes from air entering