A single layer of membrane enclosing fluid in a sac
Animal cells have many small vacuoles that are often not visible
Plant cells have one central vacuole that is visible under a microscope
Contain some substances
Remove unwanted substances from cell
Maintaining internal fluid pressure (turgor) within the cell
When bacteria are "engulfed," a portion of the cell membrane turns inside out and forms a vacuole inside the cell until the engulfed object is digested