-OriC methylated by Dam methylase at the N6 position of adenine within a palindromic sequence GATC
-Only the parent strand is methylated, but DNA replication generates two hemimethylated daughter strands
-the protein SeqA binds hemimethylated OriC and sequesters it to the plasma membrane
- OriC is released from the plasma membrane after some time at which point Dam methylase methylates the daughter strand
-Both strands must be fully methylated before DNaA can bind and initiate a new round of replication