1.Pour equal amount of starch,amylase and buffer solution to each test tube.
2.Place the test tube in a water bath.
3.After a while, add all of the solutions together and let it sit in the water.
4.Using a pipette, add a drop of the solution into the spotting tile which has iodine solution.
5.Add drops to next holes in the spotting tile every 30s, measure with a timer.
6.When the solution in the spotting tile doesnt turn black/blue but stays orange it means starch is no longer present and that amylase broke it down?