practicals

• Effect of pH on amylase. what do we need

  -Iodine solution

  -starch solution

  -buffer solution

  -amylase solution

  -test tubes

  -pipettes

  -water in a beaker

  -spotting tile
• Steps

  1.Pour equal amount of starch,amylase and buffer solution to each test tube.

  2.Place the test tube in a water bath.

  3.After a while, add all of the solutions together and let it sit in the water.

  4.Using a pipette, add a drop of the solution into the spotting tile which has iodine solution.

  5.Add drops to next holes in the spotting tile every 30s, measure with a timer.

  6.When the solution in the spotting tile doesnt turn black/blue but stays orange it means starch is no longer present and that amylase broke it down?
• Why is water bath needed

  To make the solutions the right temperature
• Whats buffer solution


  A solution that controls pH
• How many of each solution (amylase,starch,buffer) do we need?

  2cm cubed