Transferring microorganisms using aseptic techniques
1. Pass metal loop through Bunsen burner flame
2. Allow metal loop to cool
3. Remove lid of culture bottle (Tube A)
4. Glide loop over agar surface without applying pressure (inoculation)
5. Replace lid of culture bottle, sweep neck through flame
6. Spread microbes over agar surface in Petri dish by gently gliding metal loop (plating)
7. Hold Petri dish lid at an angle rather than removing completely
8. Heat metal loop again to high temp to destroy any remaining microorganisms
9. Tape Petri dish 3-4 times and incubate at 25C