any one of many specialized organic substances, composed of polymersaminoacids, that acts as catalysts to regulate the speed of the many chemical reactions involved in the metabolism of living organisms
enzymes
it was suggest in 1867
enzyme
german physiologist
willhelm kühne (1836-1900)
derived from the greek phase, meaning “inleaven”
enzymē
substance acted upon by the enzyme
substrate
what are the types of reaction that can be catalyzed
oxidoreductases
transferases
hydrolases
lyases
isomerase
ligasesorsynyhetase
catalyzed the transfer of a group of atoms
transferases
catalyzed oxidation and reductions
oxidoreductases
catalyze hydrolysis reaction
hydrolases
catalyze the addition of a group to a double bond or the removal of two groups from adjacent atoms to create a double
lyases
catalyze isomerization reactions
isomerase
catalyze the joining of two molecules
ligases or synyhetase
example of a common name
ptyalin
common terms in enzyme chemistry
cofactors
coenzyme
substrate
active site
activation
inhibition
competitiveinhibition
noncompetitiveinhibition
nonprotein part of an enzyme necessary for its catalytic function
cofactor
protein portion of the enzyme
apoenzyme
a nonprotein organic molecule, frequently a Bvitamin, that acts as a cofactor
coenzyme
the compound on which the enzyme works and whose reaction it speeds up
substrate
a three-dimensional cavity of the enzyme with specific chemical properties that enable it to accommodate the substrate
active site
any process that makes ak active enzyme less active or inactive
inhibition
an enzyme regulation in which an inhibitor competes with the substrate for the active site
competitive inhibition
an enzyme regulation in which an inhibitor binds to the enzyme outside of the active site, thereby changing the shape of the active site and reducing its catalytic activity
noncompetitive enzyme
apoenzyme (protein) + coenzyme (activator)
haloenzyme
what are the two composition of enzymes
simple proteins
conjugated protein
consists entirely of amino acid units
simple proteins
examples of simple proteins
pepsin,trypsin,ribonuclease
components of conjugated proteins
apoenzyme
coenzyme
protein part. inactive by itself
apoenzyme
nonprotein moiety, the activator
coenzyme
the enzyme has its particular shape necessary to maintain the active site
lock and key theory
what are the specificity of enzyme action
absolute specificity
stereochemical specificity
group specificity
linkage specificity
catalyze a particular reaction for one particular substrate only and will have no catalytic effect on substrate which are closely related
absolutespecificity
example for absolute specificity, only for urea; not for methyl urea, not for buiret
urease
catalyze stereochemical reactions
stereochemical specificity
example of stereochemical specificity
acid dehydrogenase
catalyze oxidation of L-lactic acid but not D-lactic acid
acid dehydrogenase
less selective and will act upon structurally similar moleculed
group specificity
example of group specificit
carboxypeptidase, aminopeptidase
the least specific, attack a particular kind of bind irrespective of the structure features in the vicinity of the linkage