micro lab exam 2

• catalase test 


  tests for breakdown of H2O2 via catalase enzyme
• urease test


  tests for breakdown of urea via urease enzyme
• oxidase test


  tests for presence of cytochrome c oxidase
• gelatinase test


  tests for breakdown of gelatin (assume collagen) via gelatinase
• mannitol salt agar (MSA) test


  tests for fermentation of mannitol
• EMB agar test 


  tests for lactose fermentation
• triple sugar iron (TSI) agar


  tests for fermentation of sucrose, glucose, lactose and AND production of H2S gas
• SIM tube


  sulfide, indole, motility
• sulfide test


  tests breakdown of cysteine via cysteine desulfurase
• motility 


  tests for presence of motility/flagella
• indole test


  tests for breakdown of tryptophan via tryptophanase
• phenol red 


  pH indicator that is yellow in acidic conditions and hot pink in basic conditions
• replicates

  used to eliminate source of error and increase accuracy
• controls

  used to eliminate sources of error and external conditions
• phenotype 


  physical/observable characteristics of an organism due to genetic make up
• BLAST


  compares nucleotide + protein sequence in data base to identify organism
• selective tests


  MSA (g+), EMB (g-), SIM (g- diagnostic)
• differential tests


  MSA, EMB, TSI