finals gen bio

Cards (64)

  • It is central to the flow of genetic information.
    RNA
  • It carries the information that specifies a protein and encodes amino acid.

    Messenger RNA (mRNA)
  • It combines with proteins to form a ribosome, the physical location of protein synthesis.

    Ribosomal RNA (rRNA)
  • These molecules are "connectors" that bind an mRNA codon at one end and a specific amino acid to the ribosome at the correct spot along the mRNA molecule.
    Transfer RNA (tRNA)
  • It means that in the new DNA molecule there is one old and one new strand.
    Semiconservative Replication
  • Enzymes that unwind "unzip" the DNA.
    Helicase
  • Prevents the two single strands from rejoining each other.
    Binding proteins
  • An enzyme that builds short complementary piece of RNA, called RNA primer attracts the DNA polymerase.

    Primase
  • Is the enzyme that adds new DNA nucleotides that are complementary to the bases on each exposed strand.

    DNA polymerase
  • An enzyme that removes each RNA primer and replaces it with correct DNA nucleotides once the new strand of DNA is in place.

    Ligase
  • -The first step in DNA replication is to 'unzip' the double helix structure of the DNA molecule
    -This is carried out by an enzyme called helicase which breaks the hydrogen bonds holding the complementary bases of DNA together (A with T, C with G).
    -The separation of the two single strands of DNA creates a 'Y' shape called a replication 'fork'. The two separated strands will act as templates for making the new strands of DNA.
    -One of the strands is oriented in the 3' to 5' direction (towards the replication fork), this is the leading strand. The other strand is oriented in the 5' to 3' direction (away from the replication fork), this is the lagging strand. As a result of their different orientations, the two strands are replicated differently:
    Steps in DNA Replication
  • -A short piece of RNA called a primer (produced by an enzyme called primase) comes along and binds to the end of the leading strand. The primer acts as the starting point for DNA synthesis.
    -DNA polymerase binds to the leading strand and then 'walks' along it, adding new complementary, nucleotide, bases (A, C, G and T) to the strand of DNA in the 5' to 3' direction.
    -This sort of replication is called continuous
    Leading Strand
  • -Numerous RNA primers are made by the primase enzyme and bind at various points along the lagging strand.
    -Chunks of DNA, called Okazaki fragments, are then added to the lagging strand also in the 5' to 3' direction.
    -This type of replication is called discontinuous as the Okazaki fragments will need to be joined up later.
    Lagging strand
  • It carries information that can be used to construct the proteins which form structures and regulate the body's activities.

    DNA
  • It is the two process of protein synthesis.
    Transcription and Translation
  • In this process the DNA message is converted into an RNA molecule.
    Transcription
  • In this process the RNA message is used to assemble amino acids into a protein chain.
    Translation
  • It is the actual construction of the proteins.
    Translation
  • There are 64 (4X4X4) possible triplet codes, but only 20 amino acids.
    Genetic Code
  • Several codons can also act as start (AUG) or stop (UAA) signals.

    True
  • It is the sequence of the genetic code.

    4 bases of DNA: A, T, C, and G.
  • Is the 3-base sequence for an amino acid.
    Codon
  • Its function is to attach to a specific amino acid and bring that amino acids to the site where polypeptides are being constructed.
    transfer RNA
  • This RNA strand is twisted and bonded into the shape seen on the right.
    One end of the molecule attached to a specific amino acid.
    The other end has an exposed sequence of 3-bases. These are called the anticodon.

    transfer RNA
  • How many kinds of tRNA must there be?
    64 anticodons
  • Are the 'decoding' units of the cell.
    Ribosomes
  • Each ribosome consists of two subunits and is an assemblage of RNA and proteins.
    Ribosomes have binding sites for both tRNA and mRNA molecules.
    ribosomal RNA (rRNA)
  • An mRNA molecule attaches to a ribosome.
    As the ribosome moves along the mRNA, 3-base codons are exposed one at a time.
    A tRNA with an anticodon that is complimentary to the codon of the mRNA temporarily bonds with the mRNA.
    The ribosome positions the molecules so that this bonding occurs.
    As the ribosome continues its journey along the mRNA additional tRNAs bring their a.a. to the site of peptide synthesis.
    True
  • The leader sequence at the 5' end of the mRNA molecule bonds with a small ribosomal subunit.
    The first mRNA codon to specify an amino acid is usually AUG, which attracts a tRNA that carries the amino acid methionine.
    This methionine signifies the start of a polypeptide.
    A large ribosomal subunit attaches to the small subunit to complete this.
    Initiation
  • A tRNA molecule carrying the second amino acid then binds to the second codon. Amino acids are then connected by a covalent bond known as a peptide bond.
    With the protein called the elongation factors, the polypeptide grows one amino acid at a time, as tRNA's continue to deliver their cargo.

    Elongation
  • Elongation halts at a "stop" codon (UGA, UAG, or UAA).
    No tRNA correspond to these stop codons. Instead, proteins called release factors bind to the stop codon, prompting the release of the last tRNA from the ribosome.
    The ribosomal subunits separate from each other and are recycled, and the new polypeptide is released.
    Termination
  • It produces multiple copies of each mRNA, and dozens of ribosomes may simultaneously bind along the length of a single mRNA molecule.
    Transcription
  • Is associated with a protein called amyloid that fold improperly and then forms an abnormal mass in brain cells.
    Alzheimer's disease
  • mad cow disease and similar conditions in sheep and humans are caused by abnormal clumps of misfolded proteins called....
    Prions in nerve cell
  • Is consist of four types of polypeptide chains (2 alpha and two beta) encoded by separate genes.
    Hemoglobin
  • This toxin occurs in the "death cup mushroom" Amanatin inhibits RNA polymerase, making transcription impossible.

    Amanatin
  • Bacteria called Corynebacterium diphtheriae secrete a toxin that causes a respiratory illness.
    Diphtheria toxin
  • Clindamicyn, chloramphenicol, tetracyclines, and gentamicin are all antibiotics that bind to bacterial ribosomes.
    Antibiotics
  • Derived from the seeds of the castor bean plant, ricin is a potent natural poison that consists of two parts. one part binds to a cell, and the other enters the cell and inhibits protein synthesis.

    Ricin
  • Fungi in the genus Fusarium produce the toxin Trichothecenes. Biological weapons. Somehow, it interferes with ribosomes.

    Trichothecenes