pH on amylase

• 1. Place one drop of iodine solution into each well of a spotting tile
• 2. Take 3 test tubes and in the first test tube add 2cm3 of starch solution. in the second test tube add 2cm3 of amylase solution and in the third test tube add 2cm3 of pH 5 buffer solution
• 3. Place all three test tubes in a water bath at 30 degrees Celsius for 10 minutes
• 4. Combine all three solutions into one test tube and mix with a stirring rod. Put it in the waterbath and start a stopwatch
• 5. After thirty seconds, use the stirring rod to transfer one drop of solution to a well in the spotting tile which contains iodine
• 6. The iodine should turn blue-black, showing that starch is present
• 7. Take a sample of every 30 seconds and continue until the iodine remains orange
• 8. When the iodine is orange, this means that there is no longer starch present (the reaction has completed)
• 9. Repeat the experiment again but use different pH buffers such as pH 6, 7 or 8