1. Measure out a set volume of the enzyme (amylase) and substrate (starch) solutions
2. Mix them together and start a timer
3. Every 10 seconds, remove a sample and test it with iodine to see if the starch has been broken down
4. Record the time taken for all the starch to be broken down
5. Repeat this using different temperatures or pH values
6. Plot the time taken against temperature or pH and identify the optimum conditions